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| Registros recuperados : 11 | |
| 1. |  | FIGUEIREDO, C. S.; MARUCCI, S. C.; TEZZA, R. I. D.; LEMOS, M. V. F.; DESIDÉRIO, J. A. Caracterização do gene vip3A e toxidade da proteína Vip3Aa50 à lagarta-do-cartucho e à lagarta-da-soja. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 9, p. 1220-1227, set. 2013. Título em inglês: Characterization of the vip3A gene and toxicity of Vip3Aa50 protein to fall armyworm and velvetbean caterpillar.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | SEBASTIÃO, I.; LEMES, A. R. N.; FIGUEIREDO, C. S.; POLANCZYK, R. A.; DESIDÉRIO, J. A.; LEMOS, M. V. F. Toxicidade e capacidade de ligação de proteínas Cry1 e receptores intestinais de Helicoverpa armigera (Lepidoptera: Noctuidae). Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 11, p. 999-1005, nov. 2015. Título em inglês: Toxicity and binding capacity of Cry1 proteins to Helicoverpa armigera (Lepidoptera: Noctuidae) intestine receptors.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | CRIALESI-LEGORI, P. C. B.; DAVOLOS, C. C.; LEMES, A. R. N.; MARUCCI, S. C.; LEMOS, M. V. F.; FERNANDES, O. A.; DESIDÉRIO, J. A. Interação de proteínas Cry1 e Vip3A de Bacillus thuringiensis para controle de lepidópteros-praga. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 2, p. 79-87, fev. 2014. Titulo em inglês: Interaction of Cry1 and Vip3A proteins of Bacillus thuringiensis for the control of lepidopteran insect pests.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 4. | | LEITE, J. P.; BARBOSA, E. G. G; MARIN, S. R. R.; MARINHO, J. P.; FUGANTI-PAGLIARINI, R.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.; DESIDÉRIO, J. A. Caracterização de plantas transgênicas de soja obtidas com a forma constitutiva do fator de transcrição AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 14, res. 6.| Biblioteca(s): Embrapa Soja. |
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| 5. | | LEITE, J. P.; BARBOSA, E. G. G; MARIN, S. R. R.; MARINHO, J. P.; FUGANTI-PAGLIARINI, R.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.; DESIDÉRIO, J. A. Caracterização de plantas transgênicas de soja obtidas com a forma constitutiva do fator de transcrição AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 5 p. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 6. | | SILVA, I. H. S.; NASCIMENTO, J. do; SANCHES, A. C.; DESIDERIO, J. A.; VALICENTE, F. H.; SOBERON, M.; POLANCZYK, R. A.; BRAVO, A. Degradation of Cry1Ac protoxin can explain the decreased susceptibility in late instars of Helicoverpa armigera. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., 2018, Gramado. Saúde, ambiente e agricultura: anais. Gramado: SEB, 2018. p. 1341.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | MARUCCI, S. C.; FIGUEIREDO, C. S.; TEZZA, R. I. D.; ALVES, E. C. da C.; LEMOS, M. V. F.; DESIDÉRIO, J. A. Relação entre toxicidade de proteínas Vip3Aa e sua capacidade de ligação a receptores intestinais de lepidópteros-praga. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 8, p. 637-648, ago. 2015. Título em inglês: Relationship between toxicity of Vip3Aa proteins and their binding capacity to intestine receptors of lepidopteran pests.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | ALVES, M. de C.; RODRIGUES, M. G. F.; ROSSI, J. R.; FERRAUDO, A. S.; CARNEIRO, A. A.; DESIDÉRIO, J. A.; FERNANDES, O. A. Toxicity of isolated from Bacillus thurigiensis berliner in the boll weevil (Anthonomus grandis Boheman, 1883) (Coleoptera: curculionidae) larvae. In: INTERNATIONAL SYMPOSIUM ON MICROBIOLOGY AND BIOTECHNOLOGY, 1., 2012, Viçosa, MG. Annals... Viçosa, MG: Universidade Federal de Viçosa, 2012. p. 121.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | ALVES, M. de C.; ROSSI, J. R.; RODRIGUES, M. G. F.; ALVEZ, E. C. da C.; FERREUDO, A. S.; LEMOS, M. V. F.; DESIDÉRIO, J. A.; FERNANDES, O. A. Identificação e caracterização de genes vip e cry coleóptero-específicos em isolados de Bacillus thuringiensis. Pesquisa agropecuária Brasileira, Brasília, DF, v. 46, n. 9, p. 1053-1060, set. 2011 Título em inglês: Identification and characterization of coleoptera?specific vip and cry genes in Bacillus thuringiensis isolates.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | LEITE, J. P.; BARBOSA, E. G. G.; MARIN, S. R. R.; MARINHO, J. P.; CARVALHO, J. F. C.; PAGLIARINI, R. F.; CRUZ, A. S.; OLIVEIRA, M. C. N.; FARIAS, J. R. B.; NEUMAIER, N.; GUIMARÃES, F. C. M.; YOSHIDA, T.; KANAMORI, N.; FUJITA, Y.; NAKASHIMA, K.; SHINOZAKI, K. Y.; DESIDÉRIO, J. A.; NEPOMUCENO, A. L. Overexpression of the activated form of the AtAREB1 gene (AtAREB1^QT) improves soybean responses to water deficit. Genetics and Molecular Research, v. 13, n. 3, p. 6272-6286, 2014.| Biblioteca(s): Embrapa Soja. |
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| 11. | | HONNA, P. T.; FUGANTI-PAGLIARINI, R.; FERREIRA, L. C.; MOLINARI, M. D. C.; MARIN, S. R. R.; OLIVEIRA, M. C. N. de; FARIAS, J. R. B.; NEUMAIER, N.; MERTZ-HENNING, L. M.; KANAMORI, N.; NAKASHIMA, K.; TAKASAKI, H.; URANO, K.; SHINOZAKI, K.; YAMAGUCHI-SHINOZAKI, K.; DESIDÉRIO, J. A.; NEPOMUCENO, A. L. Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance. Molecular Breeding, v. 36, n. 11, nov. 2016.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 11 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
18/11/2016 |
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Data da última atualização: |
10/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
HONNA, P. T.; FUGANTI-PAGLIARINI, R.; FERREIRA, L. C.; MOLINARI, M. D. C.; MARIN, S. R. R.; OLIVEIRA, M. C. N. de; FARIAS, J. R. B.; NEUMAIER, N.; MERTZ-HENNING, L. M.; KANAMORI, N.; NAKASHIMA, K.; TAKASAKI, H.; URANO, K.; SHINOZAKI, K.; YAMAGUCHI-SHINOZAKI, K.; DESIDÉRIO, J. A.; NEPOMUCENO, A. L. |
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Afiliação: |
PATRICIA T. HONNA, UNESP; RENATA FUGANTI PAGLIARINI, CAPES; LEONARDO C. FERREIRA, CNPq; MAYLA D. C. MOLINARI, UEL; SILVANA REGINA ROCKENBACH MARIN, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; NORMAN NEUMAIER, CNPSO; LILIANE MARCIA MERTZ HENNING, CNPSO; NORIHITO KANAMORI, JIRCAS; KAZUO NAKASHIMA, JIRCAS; HIRONORI TAKASAKI, RIKEN Center for Sustainable Resource Science; KAORU URANO, RIKEN Center for Sustainable Resource Science; KAZUO SHINOZAKI, RIKEN Center for Sustainable Resource Science; KAZUKO YAMAGUCHI-SHINOZAKI, University of Tokyo; JANETE A. DESIDÉRIO, UNESP Jaboticabal; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Molecular Breeding, v. 36, n. 11, nov. 2016. |
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ISSN: |
1572-9788 |
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DOI: |
10.1007/s11032-016-0570-z |
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Idioma: |
Inglês |
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Conteúdo: |
Water deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol synthase (GolS, EC 2.4.1.123) catalyzes the first step in the biosynthesis of RFOs. In our study, soybean events overexpressing 35S:AtGolS2 were molecularly, physiological, and agronomical characterized, under drought simulated in greenhouse and in field conditions during the crop season 2014/2015. The conventional soybean cultivar BRS 184 was transformed and five positive events were obtained. Four events transmitted the transgene to further generations and in the events 2Ia1 and 2Ia4, two to four copies of AtGols2 gene were observed. Results in greenhouse showed that the overexpression of AtGolS2 in genetically modified (GM) plants led to increased galactinol transcripts, probably resulting in changes in carbohydrate metabolism. Accumulation of these transcripts that may have acted as osmoprotectors, lead to higher drought tolerance and survival rate of 2Ia4 plants. In addition, in field conditions, higher yield was observed for 2Ia4 plants under irrigated (IRR) and non-irrigated (NIRR) treatments. This result can be due to the increased synthesis of RFOs even under well-watered conditions. This field screening showed promising results for drought tolerance, suggesting that 2Ia4 plants may be useful in a breeding program for the development of drought-tolerant plants. However, additional studies are needed in further crop seasons and other sites to better characterize how these plants may outperform the WT plants under water deficit. MenosWater deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol synthase (GolS, EC 2.4.1.123) catalyzes the first step in the biosynthesis of RFOs. In our study, soybean events overexpressing 35S:AtGolS2 were molecularly, physiological, and agronomical characterized, under drought simulated in greenhouse and in field conditions during the crop season 2014/2015. The conventional soybean cultivar BRS 184 was transformed and five positive events were obtained. Four events transmitted the transgene to further generations and in the events 2Ia1 and 2Ia4, two to four copies of AtGols2 gene were observed. Results in greenhouse showed that the overexpression of AtGolS2 in genetically modified (GM) plants led to increased galactinol transcripts, probably resulting in changes in carbohydrate metabolism. Accumulation of these transcripts that may have acted as osmoprotectors, lead to higher drought tolerance and survival rate of 2Ia4 plants. In addition, in field conditions, higher yield was observed for 2Ia4 plants under irrigated (IRR) and non-irrigated (NIRR) treatments. This result can be due to the increased synthesis of RFOs even under well-watered conditions. This field screening showed promising results for drought tolerance, suggesting that... Mostrar Tudo |
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Palavras-Chave: |
Estresse abiótico. |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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Marc: |
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520 $aWater deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol synthase (GolS, EC 2.4.1.123) catalyzes the first step in the biosynthesis of RFOs. In our study, soybean events overexpressing 35S:AtGolS2 were molecularly, physiological, and agronomical characterized, under drought simulated in greenhouse and in field conditions during the crop season 2014/2015. The conventional soybean cultivar BRS 184 was transformed and five positive events were obtained. Four events transmitted the transgene to further generations and in the events 2Ia1 and 2Ia4, two to four copies of AtGols2 gene were observed. Results in greenhouse showed that the overexpression of AtGolS2 in genetically modified (GM) plants led to increased galactinol transcripts, probably resulting in changes in carbohydrate metabolism. Accumulation of these transcripts that may have acted as osmoprotectors, lead to higher drought tolerance and survival rate of 2Ia4 plants. In addition, in field conditions, higher yield was observed for 2Ia4 plants under irrigated (IRR) and non-irrigated (NIRR) treatments. This result can be due to the increased synthesis of RFOs even under well-watered conditions. This field screening showed promising results for drought tolerance, suggesting that 2Ia4 plants may be useful in a breeding program for the development of drought-tolerant plants. However, additional studies are needed in further crop seasons and other sites to better characterize how these plants may outperform the WT plants under water deficit.
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773 $tMolecular Breeding$gv. 36, n. 11, nov. 2016.
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